구강병소에서 관찰되는 다핵거대세포의 면역조직화학적 특성 평가
Immunohistochemical Evaluation of Multinucleated Giant Cells in Oral Lesions
임종호, 이지혜, 신홍인,
소속 상세정보
임종호 ( Lim Jong-Ho ) - 경북대학교 대학원 구강병리학교실
이지혜 ( Lee Ji-Hye ) - 매사추세츠 공과대학 피코우어 학습-기억연구소
신홍인 ( Shin Hong-In ) - 경북대학교 대학원 구강병리학교실
KMID : 0829220120360020077
Abstract
Multinucleated giant cells appear in a variety forms in different types of oral lesion. However, their nature is still not well understood. Thus, to address this issue, the immunohistochemical characteristics of inflammatory giant cells (Langhans’ giant cells in lesions of tuberculosis and foreign body giant cells in odontogenic keratocysts and squamous cell carcinomas) and tumor giant cells in central giant cell granulomas were compared with those of osteoclasts, the normal giant cell, using a panel of macrophage and osteoclast marker antibodies, such as calcitonin receptor (CT-R), c-Src, Cathepsin K (Cath-K), CD14, RANK, and c-fms. The foreign body giant cells around cholesterol clefts in inflamed odontogenic keratocysts revealed more macrophage-like characteristics than the foreign body giant cells resorbing keratin pearls in squamous cell carcinomas. As such, both cases of foreign body giant cell exhibited immunoreactivity for the macrophage markers, such as CD14, RANK, and c-fms, yet only the latter case exhibited immunoreactivity for the osteoclast markers, such as CT-R and c-Src. Moreover, both cases of foreign body giant cells were positive for TRAP activity, yet negative for Cathepsin K activity. In contrast, the other inflammatory giant cells, Langhans’ giant cells, exhibited immunoreactivity for both the macrophage and osteoclast markers, yet were negative for TRAP activity. Meanwhile, the giant cells in the central giant cell granulomas reacted positively to both the macrophage and osteoclast markers, and were also positive for TRAP activity. Accordingly, these findings suggest that the immunoprofiles of giant cells in oral lesions vary according to the nature of the lesion, despite shared osteoclast and macrophage characteristics. Furthermore, the giant cells in tumorous lesions closely associated with bony destruction revealed more osteoclastic characteristics and their enzyme components were different according to the nature of the lesion.
키워드
Immunohistochemistry; Multinucleated giant cells; Oral lesions
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